CELLYTICTM NUCLEARTM EXTRACTION KIT PDF

Article Title: Gene optimization leads to robust expression of human respiratory syncytial virus nucleoprotein and phosphoprotein in human cells and induction of humoral immunity in mice. Figure Lengend Snippet: Western blot analysis of N and P proteins in cytoplasmic and nuclear protein extracts. Cells were transfected with plasmids containing optimized N gene pNopt , optimized P gene pPopt or the empty plasmid pShuttle. Polyclonal antibodies against N and P proteins previously obtained Simabuco et al.

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Click HERE for a description of what fees might be charged. General description: The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer. Application: NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice.

It was also used to test the therapeutic potential of andrographolide for treating endometriosis. Other Notes: Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells.

All reagents necessary for extraction are included. Don't have a web profile? Create one now. Log In or Create a Profile.

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Linkage: Recommended Antibodies for Immunodetection L, N, AMAB Other Notes: Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells.

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