Servicios Personalizados Revista. Citado por SciELO. Similares en SciELO. The effects of several components of the medium, such as carbon and nitrogen sources that ensure bacterial nutritional needs, were studied. The operation parameters, such as temperature, shaking and aeration, were set and the optimum values of cell growth and specific pDNA productivity in culture were determined.
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Fill out the form below to receive a free trial or learn more about access :. We recommend downloading the newest version of Flash here, but we support all versions 10 and above. If that doesn't help, please let us know. Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help. An unexpected error occurred. Issue 6 doi: Zhang, S. Click here for the english version.
For other languages click here. Por lo general, toma menos de 2 horas para terminar todo el procedimiento. You must be signed in to post a comment.
Please sign in or create an account. By using this kit, what can I do if I'd like to increase the yield of plasmid with a low copy number? What is the maximum volume of culture that can be harvested? Thank you very much. Your demonstration is very good but you didn't mentioned the volumes of buffer added and the speed and time of centrifuge. Thanks for doing that video. There's not a lot of theory in there, but it is surely helpful for beginners.
I'm holding onto it for future coworkers. To learn more about our GDPR policies click here. If you want more info regarding data storage, please contact gdpr jove. Please input your email address below and we will manually send you an email to verify the address. Login processing JoVE Journal Biology.
This is a sample clip. Sign in or start your free trial. Your institution must subscribe to JoVE's Biology section to access this content. A very helpful and clear demonstration. With this kit is it best to do an alcohol precipitation after or unnecessary? Thank you. Immunology and Infection. Continue with Shibboleth or Forgot Password? Please enter your email address so we may send you a link to reset your password.
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Vacunas de ADN Plasmídico: Una Herramienta Terapéutica Emergente
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Purificación del ADN plásmido de las colonias de bacterias Utilizando el kit de Qiagen Miniprep
Context : The incidence of solar ultraviolet radiation UV on Earth has increased due to diminish of the ozone layer. This enviromental agent is highly genotoxic causing numerous damage in DNA molecule. Nowadays there is a growing interest in the search of compounds capable to minimize these effects. In particular, phytocompounds have been tested as excelent candidates for their antigenotoxic properties. Methods : The cell-free plasmid DNA assay was employed. The forms of plasmid were separated electrophoretically in agarose gel. For genotoxic and photoprotective evaluation of P.
Purificación y análisis de ADN y ARN